Figure 2
From: Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling
Schematic representation of the poly(A) method used to study miRNA expression. After polyadenylation, the reverse transcription of miRNA is carried out using a universal RT primer that contains degenerate nucleotides at 3' end followed by an oligo(dT) and universal reverse primer sequence. The cDNA is amplified with either (A) specific forward and universal reverse primers or (B) LNA-integrated specific forward and specific reverse primers. The amplification products are detected by SYBR Green I.