Morphological and microsatellite DNA diversity of Nigerian indigenous sheep

Table 13 Microsatellite primer sequences that were used and their base lengths

	Name	Sequence	Base length	Annealing temperature (°C)	Allele range (bp)	Dye
1	OarFCB193	TTCATCTCAGACTGGGATTCAGAAAGGC	28	54	96-136	6FAM
		GCTTGGAAATAACCCTCCTGCATCCC	26
2	OarJMP29	GTA TAC ACG TGG ACA CCG CTT TGT AC	26	56	96-150	NED
		GAA GTG GCA AGA TTC AGA GGG GAA G	25
3	OarJMP58	GAAGTCATTGAGGGGTCGCTAACC	24	58	145-169	6FAM
		CTTCATGTTCACAGGACTTTCTCTG	25
4	OarFCB304	CCCTAGGAGCTTTCAATAAAGAATCGG	27	56	150-188	6FAM
		CGCTGCTGTCAACTGGGTCAGGG	23
5	OarAE129	AATCCAGTGTGTGAAAGACTAATCCAG	27	54	133-159	6FAM
		GTAGATCAAGATATAGAATATTTTTCAACACC	32
6	BM8125	CTCTATCTGTGGAAAAGGTGGG	22	50	110-130	VIC
		GGGGGTTAGACTTCAACATACG	22
7	OarFCB128	ATTAAAGCATCTTCTCTTTATTTCCTCGC	29	55	96-130	VIC
		CAGCTGAGCAACTAAGACATACATGCG	27
8	OarCP34	GCTGAACAATGTGATATGTTCAGG	24	50	112-130	VIC
		GGGACAATACTGTCTTAGATGCTGC	25
9	OarVH72	GGCCTCTCAAGGGGCAAGAGCAGG	24	57	121-145	VIC
		CTCTAGAGGATCTGGAATGCAAAGCTC	27
10	OarHH47	TTTATTGACAAACTCTCTTCCTAACTCCACC	31	58	130-152	VIC
		GTAGTTATTTAAAAAAATATCATACCTCTTAAGG	34
11	DYMS1	AACAACATCAAACAGTAAGAG	21	59	159-211	NED
		CATAGTAACAGATCTTCCTACA	22
12	SRCRSP1	TGC AAG AAG TTT TTC CAG AGC	21	54	116-148	NED
		ACC CTG GTT TCA CAA AAG G	19
13	SRCRSP5	GGA CTC TAC CAA CTG AGC TAC AAG	24	56	126-158	NED
		GTT TCT TTG AAA TGA AGC TAA AGC AAT GC	29
14	SRCRSP9	AGA GGA TCT GGA AAT GGA ATC	21	55	99-135	6FAM
		GCA CTC TTT TCA GCC CTA ATG	21
15	MCM140	GTT CGT ACT TCT GGG TAC TGG TCT C	25	60	167-193	NED
		GTC CAT GGA TTT GCA GAG TCA G	22

ISSN: 2049-1891