Table 5 Comparative metabolism of glucose by embryos produced in vitro (IVM/IVF), obtained by flushing the uterus and used immediately (fresh) or obtained from the uterus and cultured in vitro for 1–3 days (fresh + IVC)

(a)Catabolic

Utilization (Ι)

CO ₂

(p moles/h)

IVM/

7.00 ± 1.50^a

8.21 ± 1.90^a

8.00 ± 0.12

9.80 ± 1.50

10.65 ± 1.40

Fresh

3.00 ± 0.55^b

4.15 ± 0.21^b

7.20 ± 0.50^a

NP

NP

Fresh+IVC

NP

NP

10.13 ± 0.38^b

9.24 ± 0.32

10.35 ± 0.75

(ΙΙ)Lactate

Production

(p moles/h)

IVM/IVF

24.80 ± 5.57^a

26.00 ± 6.30^a

43.94 ± 7.60^b

69.14 ± 4.50^a

66.14 ± 8.63

Fresh

10.82 ± 1.40^b

10.02 ± 1.43^b

20.60 ± 5.97^a

NP

NP

Fresh+IVC

NP

NP

38.54 ± 1.55^b

39.52 ± 8.65^b

54.13 ± 20.94

(b)Incorporation

(p mole/h)

IVM/IVF

17.70 ± 4.13

18.00 ± 5.07

14.92 ± 2.04^a

21.71 ± 4.52

21.88 ± 2.01

Fresh

13.66 ± 1.76

15.50 ± 0.73

17.00 ± 0.58^a

NP

NP

Fresh+IVC

NP

NP

43.43 ± 10.67^b

27.96 ± 3.70

30.21 ± 9.96