Table 1 Nucleotide sequences of specific primers and real-time PCR conditions for satellite cells
Target genes | GenBank accession no. | Primer sequences |
|---|---|---|
β-actin | GenBank NM205518 | F: 5′- tgcgtgacatcaaggagaag −3′ |
|  |  | R: 5′- tgccagggtacattgtggta −3′ |
GH-R | GenBank NM_001001293 | F: 5′- aacgaggacacttacttcaccaca −3′ |
|  |  | R: 5′- gcatttccatacttggggtttct −3′ |
IGF-IR | GenBank AJ223164 | F: 5′- gcagaggagagtgaggtggaa −3′ |
|  |  | R: 5′- gtaaaaggctggagatgggaga −3′ |
IGF-I | GenBank M32791 | F: 5′- tgtgctccaataaagccacct −3′ |
|  |  | R:5′- tttctgtttcctgtgttccctctac −3′ |
TRα | GenBank NM_205313 | F: 5′-tctgcgtggataagatagagaagtg-3′ |
|  |  | R: 5′- gttgtgtttgcggtagttgatgtag −3′ |
Bcl-2 | GenBank D11381 | F: 5′- gccccccgcctcaccatg −3′ |
|  |  | R: 5′- cccggggtgagccatggtttc −3′ |
Bax | GenBank NM_007527 | F: 5′- acagggtttcatccaggatcgagca-3′ |
|  |  | R: 5′- tcagcttcttggtggacgcatc −3′ |