Figure 2

Viability of satellite cells according to feeding group at 15 days of age. Con: control group; IF: intermittent feeding group; RF: re-feeding group. Cells were seeded at 10⁴ cells per well in a 96-well plate, 6 wells for each group and each day. Cell viability was assessed by the MTT assay as OD value. The absorbance was read on ELISA plate reader at 570 nm. Values are means ± SEM. Groups not sharing a common letter within the same figure are significantly different (A–C: significance within the same group; x–z: significance within the same time). P<0.05, N = 6/group/day, N is the repeat number in cell culture for one feeding group in one day per time, the experiment was repeated three times.