Figure 1
Morphologic development of the satellite cells in culture according to feeding group at 15 days of age. Optical phase contrast images in Con (control group), IF (intermittent feeding group) and RF (re-feeding group), bar: 50 μm. Cells were seeded at 5×104 cells/cm2 in standard serum-rich medium in 6-well plates (34.8-mm petri dishes). Morphological changes were followed during the first 3 days to reflect cell proliferation and differentiation capacity.