Identification of an intestine-specific promoter and inducible expression of bacterial α-galactosidase in mammalian cells by a lac operon system

Table 1 Primers and regulatory element sequences used in this paper

Name¹	GenBank	Primer sequenc (5’-3’)	Product (bp)
HMUC2	U67167	S:5’-CTAGCTAGC TCCTCCCAGCGTAACGTGAGC-3’	466
HMUC2	U67167	A:5’-GAAGATCT CTAGTGGCAGCCCCATGGTG-3’	466
HIFABP	NG_011444	S:5’-CCGACGCGT GTTAGATTTATCTTCCCTTGACC-3’	1154
HIFABP	NG_011444	A:5’-CCGCTCGAG TACCTTCCAAGTGCTGTCAAAC-3’	1154
RIFABP	NW_047627	S:5’-CGACGCGT CATGCTGAATTCCTTAATTTGC-3’	1232
RIFABP	NW_047627	A:5’-CCGCTCGAG CAGCTGTGTGTGCCTCTAGG-3’	1232
HLY	NM_000239	S:5’-CTAGCTAGC CTGTCCTCTTAGGCAGATACAGA-3’	1186
HLY	NM_000239	A:5’- GAAGATCT AGAGCCTTCATGTTGACTGCTA-3’	1186
HSI	X85797	S: 5’- GGGGTACC CAATGAGTGCTATCTGTGGT-3’	230
HSI	X85797	A: 5’-CGACGCGT AAGGAAAGCTGCTTAGGTA-3’	230
LacI	V00294	S: 5'-CCCAAGCTT CCGGAAGAGAGTCAATTCAG-3'	1143
LacI	V00294	A:5'-GCTCTAGA AGTTTCGAAGGAGAAGAAGAATCCCTGCCCGCTTTCCAGTC'-3’	1143
GFP	AY268072.1	S: 5'-CCAAGCTT TTGTTTCGTTTTCTGTTCTGCG-3'	763
GFP	AY268072.1	A: 5'-GCTCTAGA GCAGCGTATCCACATAGCGTAA-3'	763
α-Gal	DQ344486	S: 5’-CATGCCATGG GCGGATGGTACTCTCTTTG-3’	2153
α-Gal	DQ344486	A: 5’-GCTCTAGA CTATTGCTTTTCCAACATCA-3’	2153
RITF enhancer		5’-CGGGGTACCTGTTTTCCTCCCTAACCCTCTCCCCTCCCCCTCGGACTCCCACGCGTCGC-3’	59

¹HMUC2 = human mucin-2 promoter; HIFABP = human intestinal fatty acid binding protein promoter; RIFABP = rat intestinal fatty acid binding protein promoter; HLY = human lysozyme promoter; HSI= human sucrase-isomaltase enhancer; LacI = lac operon repressor; GFP = green fluorescent protein; α-Gal = α-galactosidase; RITF = rat intestinal trefoil factor.

ISSN: 2049-1891