Effects of inducers on the transient expression of α-galactosidase (α-Gal) mRNA and on α-Gal activity levels. (A) RT-PCR analysis of α-Gal mRNA levels; β-actin was used to control transfection efficiency and cell recovery. (B) α-Gal activity assay. Values are the average of triple determinations with the SD indicated by error bars. Different letters represent significant difference, P < 0.01.