Effects of

Table 1 Primer sequences and annealing temperatures

Gene	Serial number	Primer sequences (5’-3’)	Annealing temp (°C)	Length (bp)
β-actin	NM_205518	FP: CACCGCAAATGCTTCTAAAC	58	100
β-actin	NM_205518	RP: GCCATGCCAATCTCGTCTT	58	100
NPY	NM_205473	FP: TGTTGAGGGAAAGCACAGAA	59	132
NPY	NM_205473	RP: GATTTGCTTCAGAGGAGTGGA	59	132
AgRP	AB489993	FP: CATCCTCACCTCGGACCTCA	63	111
AgRP	AB489993	RP: GGGCCATCTGATCCAAGTCT	63	111
POMC	NM_001031098	FP: AGAAGGGTTGGAACGAGA	61	99
POMC	NM_001031098	RP: TACACCTTGATGGGTCTCC	61	99
MC4R	NM_001031514	FP: TGGAACCAGAGCAACGGAC	62	156
MC4R	NM_001031514	RP: TGCCACAATGACCAAGACG	62	156
CRF	AJ621492	FP: TCCCTGGACCTGACTTTCC	58	117
		RP: GCCTCACTTCCCGATGATTT

ISSN: 2049-1891