Table 1 Primer and probe sequences, dual labeling (only for probes), and position in the reference sequences in real-time PCR-based assays for CVM and BLAD
Genetic defect | Molecule | Oligo sequence, 5′ to 3′ | Reporter, 5′ | Quencher, 3′ | Position1 |
|---|---|---|---|---|---|
CVM | Â | Â | Â | Â | Â |
| Â | Primers | Â | Â | Â | Â |
|  |  SLC35A3-FWD | AGCTGGCTCACAATTTGTAGGT |  |  | 9840-9861 |
|  |  SLC35A3-REV | CTCAAAGTAAACCCCAGCAAAGC |  |  | 9896-9918 |
| Â | Probes | Â | Â | Â | Â |
|  |  SLC35A3-WD | TCATGGCAGTTCTCA | VIC | NFQ | 9863-9877 |
|  |  SLC35A3-MUT | TCATGGCATTTCTCA | FAM | NFQ | 9863-9877 |
BLAD | Â | Â | Â | Â | Â |
| Â | Primers | Â | Â | Â | Â |
|  |  CD18-FWD | GTTGCGTTCAACGTGACCTT |  |  | 1154-1173 |
|  |  CD18-REV | GAGTAGGAGAGGTCCATCAGGTA |  |  | 1208-1230 |
| Â | Probes | Â | Â | Â | Â |
|  |  CD18-WD | CCCCATCGACCTGTAC | VIC | NFQ | 1192-1207 |
|  |  CD18-MUT | CCCATCGGCCTGTAC | FAM | NFQ | 1193-1207 |