Excess dietary cholesterol may have an adverse effect on growth performance of early post-larval Litopenaeus vannamei

Table 3 Total lipid and lipid class of whole body shrimp fed experimental diets

Cholesterol levels, %	D10	D20.5	D31	D42	One way ANOVA (P value)
Lipid composition
Total lipid¹	1.6 ± 0.1^b	1.7 ± 0.1^b	1.7 ± 0.1^b	2.2 ± 0.1^a	<0.001
Neutral lipid ²	35.1 ± 1.0^b	36.2 ± 3.0^b	37.7 ± 1.7^b	46.6 ± 3.9^a	0.008
TC	20.9 ± 0.3^b	22.4 ± 2.8^b	23.3 ± 1.3^b	27.7 ± 3.1^a	0.043
TG	0.7 ± 0.03	0.6 ± 0.02	0.7 ± 0.04	0.7 ± 0.02	0.986 (ns)
FFA	9.3 ± 0.2^c	11.7 ± 0.5^b	14.8 ± 1.1^a	16.2 ± 0.2^a	<0.001
Polar lipid	64.9 ± 1.1^a	63.8 ± 3.0^a	62.3 ± 1.7^a	53.4 ± 3.9^b	0.009
PC	40.4 ± 0.5	40.6 ± 1.0	40.1 ± 1.3	37.0 ± 1.4	0.153 (ns)
PE	14.6 ± 0.2^a	12.5 ± 1.1^ab	10.7 ± 0.3^b	8.5 ± 0.6^c	0.001
PI	0.52 ± 0.02	0.49 ± 0.01	0.53 ± 0.03	0.52 ± 0.05	0.835 (ns)
Others	9.4 ± 0.5	10.2 ± 1.3	11.1 ± 0.6	7.4 ± 2.1	0.311 (ns)

Values are shown as means ± SE of three replicates. Means within the same row and not sharing a common superscript are significantly different (Ducans, P < 0.05); ns, no significant difference detected (P > 0.05).
TC = total cholesterol; TG = triglycerides; FFA = free fatty acids; PC = phosphatidylcholine; PE = phosphatidylethanolamine; PI = phosphatidylinositol.
¹% Wet weight.
²% Total lipid.