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Table 1 Ingredients and proximate composition of experimental diets (% dry matter)

From: Excess dietary cholesterol may have an adverse effect on growth performance of early post-larval Litopenaeus vannamei

Ingredients

D1

D2

D3

D4

 White fish meal

50.75

50.75

50.75

50.75

 Protein hydrolysate

20

20

20

20

 a-Starch

5

5

5

5

 Soybean oil

3

3

3

3

 Phospholipid (purity 97%, pc-60)

2

2

2

2

 Vitamin premix1

1

1

1

1

 Mineral premix2

4

4

4

4

 Vitamin C

0.65

0.65

0.65

0.65

 Krill meal

3

3

3

3

 Beer yeast

3

3

3

3

 Cellulose

2

1.5

1.0

0

 Cholesterol (purity 95%)

0

0.5

1

2

 Others3

5.6

5.6

5.6

5.6

Proximate composition

    

 Moisture

7.30

7.27

5.84

5.09

 Cholesterol

0.92

1.32

1.80

2.75

 Crude protein

57.6

57.8

57.8

57.8

 Crude lipid

12.2

12.2

13.0

12.8

 Ash

17.0

17.0

17.1

17.0

  1. 1Contents (g/100 g) retinyl acetate, 0.25; cholecalciferol, 0.625; all-rac-a-tocopheryl acetate, 7.5; menadione, 0.25; thiamin, 0.025; riboflavin, 0.1; D-calcium pantothenate, 0.5; pyridoxine HCL, 0.075; cyanocobalamin, 0.25; niacin, 0.25; folic acid, 0.025; biotine, 0.25; meso-inositol, 37.9; cellulose, 50. (Niu et al. 2008) [10].
  2. 2Contents (g/100 g) KCL, 9; KI, 4 mg; NaCL, 4; CuSO4-5H2O, 0.3; ZnSO4-7H2O, 0.4; CoSO4-7H2O, 2 mg; FeSO4-7H2O, 2; MnSO4-H2O, 0.3; MgSO4-7H2O, 12.4; Ca(HPO4)2-2H2O, 50; CaCO3, 21.5. (Niu et al. 2008) [10].
  3. 3Contents (g/100 g): Sodium alginate, 3; Choline chloride, 1; Methionine, 1; Tryptophan, 0.6.