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Table 1 Ingredients and proximate composition of experimental diets (% dry matter)

From: Excess dietary cholesterol may have an adverse effect on growth performance of early post-larval Litopenaeus vannamei

Ingredients D1 D2 D3 D4
 White fish meal 50.75 50.75 50.75 50.75
 Protein hydrolysate 20 20 20 20
 a-Starch 5 5 5 5
 Soybean oil 3 3 3 3
 Phospholipid (purity 97%, pc-60) 2 2 2 2
 Vitamin premix1 1 1 1 1
 Mineral premix2 4 4 4 4
 Vitamin C 0.65 0.65 0.65 0.65
 Krill meal 3 3 3 3
 Beer yeast 3 3 3 3
 Cellulose 2 1.5 1.0 0
 Cholesterol (purity 95%) 0 0.5 1 2
 Others3 5.6 5.6 5.6 5.6
Proximate composition     
 Moisture 7.30 7.27 5.84 5.09
 Cholesterol 0.92 1.32 1.80 2.75
 Crude protein 57.6 57.8 57.8 57.8
 Crude lipid 12.2 12.2 13.0 12.8
 Ash 17.0 17.0 17.1 17.0
  1. 1Contents (g/100 g) retinyl acetate, 0.25; cholecalciferol, 0.625; all-rac-a-tocopheryl acetate, 7.5; menadione, 0.25; thiamin, 0.025; riboflavin, 0.1; D-calcium pantothenate, 0.5; pyridoxine HCL, 0.075; cyanocobalamin, 0.25; niacin, 0.25; folic acid, 0.025; biotine, 0.25; meso-inositol, 37.9; cellulose, 50. (Niu et al. 2008) [10].
  2. 2Contents (g/100 g) KCL, 9; KI, 4 mg; NaCL, 4; CuSO4-5H2O, 0.3; ZnSO4-7H2O, 0.4; CoSO4-7H2O, 2 mg; FeSO4-7H2O, 2; MnSO4-H2O, 0.3; MgSO4-7H2O, 12.4; Ca(HPO4)2-2H2O, 50; CaCO3, 21.5. (Niu et al. 2008) [10].
  3. 3Contents (g/100 g): Sodium alginate, 3; Choline chloride, 1; Methionine, 1; Tryptophan, 0.6.