Table 1 Primer sequences of chicken cathelicidins for real time PCR
From: Tissue expression and developmental regulation of chicken cathelicidin antimicrobial peptides
Gene | Forward primer (5' to 3') | Reverse primer (5' to 3') | Product size, bp | |
|---|---|---|---|---|
| Â | Â | Â | cDNA | Gene |
Fowlicidin-1 | GCTGTGGACTCCTACAACCAAC | GGAGTCCACGCAGGTGACATC | 261 | 882 |
Fowlicidin-2 | CAAGGAGAATGGGGTCATCAG | CGTGGCCCCATTTATTCATTCA | 221 | 584 |
Fowlicidin-3 | GCTGTGGACTCCTACAACCAAC | TGGCTTTGTAGAGGTTGATGC | 352 | 1095 |
Cathelicidin B1 | CCGTGTCCATAGAGCAGCAG | AGTGCTGGTGACGTTCAGATG | 170 | 251 |
GAPDH | GCACGCCATCACTATCTTCC | CATCCACCGTCTTCTGTGTG | 356 | 876 |