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Fig. 6 | Journal of Animal Science and Biotechnology

Fig. 6

From: High expression circRALGPS2 in atretic follicle induces chicken granulosa cell apoptosis and autophagy via encoding a new protein

Fig. 6

CircRALGPS2-212aa promoted chicken granulosa cell apoptosis and autophagy. A The protein abundance and gray value statistics of caspase 3, caspase 9, Beclin 1 and LC3 in granulosa cells after transfection with ov-circRALGPS2-FLAG plasmid. B The protein abundance and gray value statistics of caspase 3, caspase 9, Beclin 1 and LC3 in granulosa cells after transfection with pcDNA3.1-circRALGPS2-FLAG plasmid. C Flow cytometry results of granulosa cells after transfection with ov-circRALGPS2-FLAG plasmid. D Flow cytometry results of granulosa cells after transfection with pcDNA3.1- FLAG plasmid. E Confocal microscopy observation of autophagosomes in granulosa cells after transfection with ov-circRALGPS2-FLAG plasmid. Green fluorescence, lysosome; red fluorescence, autophagosome; yellow fluorescence, autophagosome. F Confocal microscopy observation of autophagosomes in granulosa cells after transfection with pcDNA3.1-circRALGPS2-FLAG plasmid. *P < 0.05, **P < 0.01. Data are presented as mean ± SEM. n = 9 for qRT-PCR, and n = 3 for Western blotting

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891