Fig. 4

Protein translation prediction of circRALGPS2. A Schematic representation of the open reading frame of circRALGPS2. B Schematic diagram of circRALGPS2 translation protein circRALGPS2-212aa, the purple fragment is the specific peptide of circRALGPS2-212aa. C Schematic diagram of a dual-luciferase reporter gene (Luc2-IRES-Reports vector) containing different fragments of circRALGPS2 for IRES validation. D The ratio of firefly luciferase to Renilla luciferase activities after transfection of the dual-luciferase reporter vector into DF-1 cells. ^**P < 0.01. Data are presented as mean ± SEM. n = 3 for Double luciferase reporting test