Investigation of HCAR2 antagonists as a potential strategy to modulate bovine leukocytes

Table 1 Expression of HCAR2 on circulating bovine immune cell populations in early and mid-lactation dairy cows (n = 6 per stage of lactation)

Item¹	Early lactation²	Mid-lactation	SEM	P-value
HCAR2⁺, % of live cells	1.71	3.53	0.849	0.09
Monocytes, % of live cells	30.03	32.82	6.715	0.75
HCAR2⁺, % of Monocytes	1.90	1.83	0.859	0.95
T-cells, % of live cells	24.26	28.97	4.005	0.34
HCAR2⁺, % of T-cells	3.24	5.37	1.030	0.09
B-cells, % of live cells	37.88	38.04	5.255	0.98
HCAR2⁺, % of B-cells	2.12	5.44	0.811	< 0.01
Granulocytes, % of live cells	26.60	28.51	6.883	0.84
HCAR2⁺, % of granulocytes	1.00	1.52	0.468	0.33

¹Monocytes = CD172a⁺, B-cells = CD21⁺, T-cells = CD3⁺, Granulocytes separated by side scatter. Cell populations do not add to 100% due to some B-cells being counted as granulocytes; this may be related to bovine leukemia virus present in this population of cows
²Early lactation cows were less than 10 d in milk and mid-lactation cows were between 115 and 200 d in milk. No cows had adverse health events in the 90 d prior to sampling

ISSN: 2049-1891