Fig. 2

BAT changes to WAT during perirenal adipose tissue development. A Morphological comparison of perirenal adipose tissue. B Changes of perirenal adipose tissue weight. C Ratio of perirenal adipose tissue to bodyweight. D H&E staining of perirenal adipose tissue. E Statistics of perirenal adipose tissue area. F–I The mRNA expression (F), immunohistochemical staining (G), and protein expression (H and I) of UCP1. J–L Relative gene expression normalized to the geometric mean of UCP2, DIO2, and PPARGC1a. M The triglyceride content. N and O Relative gene expression normalized to the geometric mean of ADIPOQ (N) and PPARG (O). P and Q Perirenal adipose tissue mtDNA content (P) and observed by TEM (Q). R–V The expression levels of mitochondrial complex proteins (complexes I–V) were measured by Western blotting. ^*P < 0.05, ^**P < 0.01