Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs

Table 1 Primer details for quantifying sperm mitochondrial DNA content (mtDNAc) by qPCR

Genome	Target	Primer sequence (5´→3´)	Tm, °C	Amplicon size, bp	Primer efficiency
Nuclear	BAX	FW: GGACTTCCTTCGAGATCGGC	60	161	94.81
Nuclear	BAX	RV: GAGCACATCTGGTGACCCAA	60	161	94.81
Mitochondrial	ND1	FW: CCATGTTCATTATTGCACCAATCC	60	190	109.88
Mitochondrial	ND1	RV: GCCCCGATGAGTGCGTATTT	60	190	109.88

Sequence, melting temperature (Tm), amplicon size and efficiency of the primers used to amplify the nuclear (BCL2 Associated X; BAX) and mitochondrial (NADH dehydrogenase subunit 1; ND1) genes. The number of copies of these genes were subsequently utilised to quantify mitochondrial DNA content (mtDNAc) in pig sperm. FW Forward; RV Reverse

ISSN: 2049-1891