Effects of dietary Clostridium butyricum and rumen protected fat on meat quality, oxidative stability, and chemical composition of finishing goats

Table 2 Primers used for quantitative real-time PCR

Genes	Primer sequence (5′→3′)	Product size, bp	GenBank No.
ACC	GGCGGGATGGTCTCTTTTC	145	DQ370054.1
ACC	TGGGGACCTTGTCTTCATCATAC	145	DQ370054.1
FAS	GGGCTCCACCACCGTGTTCCA	226	NM_001285629.1
FAS	GCTCTGCTGGGCCTGCAGCTG	226	NM_001285629.1
LPL	GGACACTTGCCACCTCA	278	NM_001285607.1
LPL	CCGCCATCCAGTTCATA	278	NM_001285607.1
SREBP-1	CTGCTGACCGACATAGAAGACAT	81	NM_001285755.1
SREBP-1	GTAGGGCGGGTCAAACAGG	81	NM_001285755.1
SCD	TGGCGTTCCAGAATGACGTT	82	NM_001285619.1
SCD	TGGGGATCAGCATCCGTTTC	82	NM_001285619.1
C/EBPα	CCGTGGACAAGAACAGCAA	141	XM_018062278.1
C/EBPα	GGCGGTCATTGTCACTGG	141	XM_018062278.1
HSL	CCTCCTCGTGGCTCAACTCCTT	195	XM_018062484.1
HSL	CTGTTGTGTCGCTGCTGTTCCT	195	XM_018062484.1
CPT1B	GTCTGGGTGATGGGCATCTTCTTC	144	NM_001009259.1
CPT1B	TCTGGTCAAGTGGCTGGTCTGG	144	NM_001009259.1
PPARγ	CCTTCACCACCGTTGACTTCT	145	NM_001285658.1
PPARγ	GATACAGGCTCCACTTTGATTGC	145	NM_001285658.1
β-actin	CTCACGGAGCGTGGCTACA	107	JX046106
β-actin	GCCATCTCCTGCTCGAAGTC	107	JX046106

ACC Acetyl-CoA carboxylase α, FAS Fatty acid synthase, LPL Lipoprotein lipase, SREBP-1 Sterol regulatory element-binding transcription factor 1, SCD Stearoyl-CoA desaturase, C/EBPα CCAAT/enhancer binding protein alpha, HSL Hormone-sensitive lipase, CPT1B Carnitine palmitoyltransferase-1B, PPARγ Peroxisome proliferators activated receptor γ

ISSN: 2049-1891