Fe source | Added Fe, mg/kg | CAT mRNA5, RQ | SDH mRNA5, RQ | COX mRNA5, RQ | ||||||
---|---|---|---|---|---|---|---|---|---|---|
Heart | Kidney | Liver | Heart | Kidney | Liver | Heart | Kidney | Liver | ||
Control1 | 0 | 1.01 | 1.02 | 1.01 | 1.01 | 1.01* | 1.03* | 1.01 | 1.00 | 1.01 |
FeSO4∙7H2O1 | 20 | 1.05 | 0.97 | 0.98 | 1.02 | 1.05 | 1.05 | 0.88 | 0.89 | 1.01 |
40 | 0.96 | 1.02 | 1.21 | 0.91 | 1.10 | 1.17 | 1.09 | 1.08 | 0.99 | |
60 | 0.95 | 0.95 | 1.19 | 1.02 | 1.19 | 1.30 | 0.84 | 0.94 | 1.16 | |
Fe-Prot ES1 | 20 | 1.13 | 0.93 | 0.99 | 0.98 | 1.16 | 1.23 | 0.91 | 0.93 | 1.08 |
40 | 1.13 | 0.90 | 1.18 | 0.98 | 1.31 | 1.36 | 1.00 | 0.94 | 1.08 | |
60 | 1.13 | 0.92 | 0.91 | 0.97 | 1.42 | 1.46 | 0.94 | 0.99 | 1.21 | |
Feed grade NaFeEDTA1 | 20 | 1.54 | 1.03 | 1.11 | 1.04 | 1.19 | 1.20 | 1.07 | 0.94 | 1.08 |
40 | 1.58 | 0.95 | 1.19 | 0.85 | 1.30 | 1.47 | 0.94 | 1.02 | 1.08 | |
60 | 1.48 | 1.11 | 1.02 | 0.94 | 1.44 | 1.51 | 1.01 | 1.12 | 1.15 | |
Food grade NaFeEDTA1 | 20 | 1.26 | 0.98 | 1.03 | 0.97 | 1.26 | 1.39 | 0.94 | 0.90 | 0.94 |
40 | 1.27 | 0.98 | 1.15 | 0.93 | 1.23 | 1.37 | 0.96 | 0.86 | 1.07 | |
60 | 1.49 | 0.91 | 0.95 | 1.00 | 1.35 | 1.51 | 0.96 | 0.85 | 1.23 | |
Pooled SE | 0.13 | 0.07 | 0.11 | 0.07 | 0.08 | 0.09 | 0.1 | 0.07 | 0.09 | |
Fe source2 | FeSO4∙7H2O | 0.99C | 0.98 | 1.13 | 0.98 | 1.11B | 1.17B | 0.94 | 0.97 | 1.05 |
Fe-Prot ES | 1.13BC | 0.92 | 1.03 | 0.98 | 1.30A | 1.35A | 0.95 | 0.95 | 1.13 | |
Feed grade NaFeEDTA | 1.53A | 1.03 | 1.11 | 0.95 | 1.31A | 1.39A | 1.01 | 1.03 | 1.10 | |
Food grade NaFeEDTA | 1.34AB | 0.96 | 1.04 | 0.97 | 1.28A | 1.42A | 0.95 | 0.87 | 1.08 | |
Pooled SE | 0.08 | 0.04 | 0.07 | 0.04 | 0.06 | 0.05 | 0.05 | 0.04 | 0.06 | |
Added Fe level3, mg/kg | 20 | 1.25 | 0.98 | 1.03 | 1.00 | 1.16b | 1.22b | 0.95 | 0.91 | 1.03b |
40 | 1.24 | 0.96 | 1.18 | 0.92 | 1.24ab | 1.34ab | 1.00 | 0.97 | 1.06ab | |
60 | 1.26 | 0.97 | 1.01 | 98 | 1.35 a | 1.45a | 0.94 | 0.97 | 1.18a | |
Pooled SE | 0.07 | 0.03 | 0.06 | 0.04 | 0.04 | 0.05 | 0.05 | 0.04 | 0.05 | |
P-value | Fe source | < 0.0001 | 0.2679 | 0.6672 | 0.9303 | 0.0143 | 0.0048 | 0.8188 | 0.0792 | 0.8131 |
Added Fe level | 0.9562 | 0.9508 | 0.0954 | 0.2172 | 0.0091 | 0.0027 | 0.6265 | 0.4327 | 0.0423 | |
Fe Source × added Fe level | 0.8794 | 0.6658 | 0.8536 | 0.8476 | 0.9340 | 0.8465 | 0.6108 | 0.5054 | 0.9464 | |
Linear effect4 | – | – | – | – | 0.0003 | 0.0001 | – | – | 0.0596 |