Fig. 7
From: miR-423 sponged by lncRNA NORHA inhibits granulosa cell apoptosis
miR-423 mediated NORHA regulation of SMAD7 levels and GC apoptosis. A–C GCs were co-transfected with the pcDNA3.1-NORHA and miR-423 mimics, and the levels of SMAD7 mRNA (A) were detected by qPCR, and the levels of SMAD7 protein (B) and p-SMAD3 protein (C) were detected by western blotting. D–G GCs were co-transfected with the pcDNA3.1-NORHA and miR-423 mimics, and apoptosis was detected by flow cytometry (D), and total apoptosis rate (E), early apoptosis rate (F) and late apoptosis rate (G) was calculated, respectively. oe, overexpression; NC, negative control. Data represented are mean ± SEM for three independent experiments. *P < 0.05