Fig. 6

NORHA is an ceRNA of miR-423 in GCs. A Schematic showing the MRE of miR-423 in the porcine NORHA transcript. B The minimal free energy (mfe) between miR-423 and NORHA tanscript was predicted by RNAhybrid. C RNA pull-down analysis of the physical interaction miR-423 and NORHA transcript in GCs. D Schematic annotation of the reporter vectors of NORHA with the wild-type and mutated MRE of miR-423. E Luciferase assay. KGN cells were co-transfected with the reporter vectors and miR-423-specific mimics, luciferase activity was determined. F–G NORHA regulates SMAD7 expression in GCs. GCs were transfected with the pcDNA3.1-NORHA, the levels of NORHA (F), and SMAD7 mRNA (G) were detected by qPCR. wt, wild type; mut, mutation; NC, negative control. Data represented are mean ± SEM for three independent experiments. ^*P < 0.05. ns, not significant