Fig. 3

SMAD7 is a potential target of miR-423. A Alignments of pre-miR-423 sequences in mammals. B Subcellular distribution of miR-423 in GCs. The levels of internal reference genes (U6 in the nucleus and GAPDH in the cytoplasm) and miR-423 were detected by qPCR, respectively. C–E Potential function prediction of miR-423. The potential targets of miR-423 were predicted by three algorithms (TarBase, miRDB, TargetScan) (C), GO (D) and KEGG (E) analysis with miR-423 targets were performed, respectively. F The minimal free energy (mfe) between miR-423 and the 3'-UTR of the porcine SMAD7 gene was predicted by RNAhybrid. The heatmap shows the −log₁₀ (P value) of the terms. Data represented are mean for three independent experiments