Fig. 2

miR-423 represses GC apoptosis. A Overexpression efficiency assay. GCs were transfected with miR-423-specific mimics, miR-423 level was detected by qPCR. B–E Detection of GC apoptosis rate. Q1, injured or dead cells. Q2, early apoptosis cells. Q3, live cells. Q4, late apoptosis cells. GCs were transfected with miR-423-specific mimics, apoptosis was detected by flow cytometry (B), and total apoptosis rate (C), early apoptosis rate (D) and late apoptosis rate (E) was calculated, respectively. NC, negative control. Data represented are mean ± SEM for three independent experiments. ^*P < 0.05. ns, not significant