Amino acid and mineral digestibility, bone ash, and plasma inositol is increased by including microbial phytase in diets for growing pigs

Lagos, Liz Vanessa; Bedford, Mike Richard; Stein, Hans Henrik

doi:10.1186/s40104-023-00953-x

Journal of Animal Science and Biotechnology

Table 2 Ingredient composition and analyzed composition of experimental diets^a

From: Amino acid and mineral digestibility, bone ash, and plasma inositol is increased by including microbial phytase in diets for growing pigs

Item	Phytase units (FTU)/kg feed
Item	0	250	500	1,000	2,000	4,000
Ingredient, %
Ground maize	49.70	49.20	49.20	49.20	49.20	49.20
Soybean meal (48% crude protein)	35.00	35.00	35.00	35.00	35.00	35.00
Lactose	10.00	10.00	10.00	10.00	10.00	10.00
Soybean oil	2.30	2.30	2.30	2.30	2.30	2.30
Calcium carbonate	0.95	0.95	0.95	0.95	0.95	0.95
Monocalcium phosphate	0.30	0.30	0.30	0.30	0.30	0.30
Sodium bicarbonate	0.32	0.32	0.32	0.32	0.32	0.32
L-Lys HCl (78% Lys)	0.28	0.28	0.28	0.28	0.28	0.28
DL-Met	0.12	0.12	0.12	0.12	0.12	0.12
L-Thr	0.08	0.08	0.08	0.08	0.08	0.08
Sodium chloride	0.40	0.40	0.40	0.40	0.40	0.40
Chromic oxide	0.40	0.40	0.40	0.40	0.40	0.40
Vitamin mineral premix^b	0.15	0.15	0.15	0.15	0.15	0.15
Phytase-corn premix^c	-	0.50	0.50	0.50	0.50	0.50
Total	100	100	100	100	100	100
Analyzed values
Gross energy, kcal/kg	3,987	3,956	4,003	3,976	3,991	3,995
Dry matter, %	87.95	87.86	87.95	87.81	87.78	87.75
Ash, %	5.35	5.04	5.06	5.22	5.27	5.28
Crude protein, %	19.42	19.59	19.40	19.07	19.72	20.16
AEE^d, %	3.72	3.77	3.96	3.74	3.83	3.93
Amino acids, %
Arg	1.38	1.30	1.32	1.29	1.28	1.34
His	0.55	0.52	0.53	0.52	0.51	0.53
Ile	0.95	0.91	0.91	0.89	0.87	0.91
Leu	1.71	1.64	1.68	1.65	1.61	1.68
Lys	1.45	1.34	1.36	1.36	1.33	1.36
Met	0.44	0.37	0.40	0.41	0.37	0.44
Phe	1.02	1.01	0.99	0.97	0.95	1.03
Thr	0.86	0.81	0.79	0.81	0.80	0.83
Trp	0.28	0.26	0.27	0.26	0.27	0.27
Val	1.04	0.98	0.99	0.97	0.96	0.99
Ca, %	0.58	0.56	0.55	0.55	0.57	0.60
P, %	0.43	0.44	0.42	0.42	0.42	0.43
Phytate bound-P, %	0.26	0.25	0.24	0.24	0.23	0.21
Phytase activity, FTU/kg	< 50	417	715	1,680	2,760	5,350
Phytate esters^e, nmol/g dry matter
IP6	15,225	12,920	13,732	15,242	14,540	12,005
IP5	1,964	1,831	1,851	2,029	1,790	1,700
IP4	244	228	275	273	282	444

^aDiets were formulated to contain the following quantities of net energy, amino acids (expressed as standardized ileal digestible), Ca, and P: net energy, 2,473 kcal/kg; Lys, 1.23%; Met, 0.40%; Thr, 0.74%; Trp, 0.23%; Ca, 0.54%; P, 0.44%
^bThe vitamin–micromineral premix provided the following quantities of vitamins and micro minerals per kg of complete diet: vitamin A as retinyl acetate, 11,150 IU; vitamin D₃ as cholecalciferol, 2,210 IU; vitamin E as DL-alpha tocopheryl acetate, 66 IU; vitamin K as menadione dimethylprimidinol bisulfite, 1.42 mg; thiamin as thiamine mononitrate, 1.10 mg; riboflavin, 6.59 mg; pyridoxine as pyridoxine hydrochloride, 1.00 mg; vitamin B₁₂, 0.03 mg; D-pantothenic acid as D-calcium pantothenate, 23.6 mg; niacin, 44.1 mg; folic acid, 1.59 mg; biotin, 0.44 mg; Cu, 20 mg as copper sulfate; Fe, 125 mg as iron sulfate; I, 1.26 mg as ethylenediamine dihydriodide; Mn, 60.2 mg as manganous sulfate; Se, 0.30 mg as sodium selenite and selenium yeast; and Zn, 125.1 mg as zinc sulfate
^cThe phytase concentrate contained 5,000 units of phytase/g (Quantum Blue, AB Vista, Marlborough, UK). Five separate premixes were prepared by mixing 80, 160, 320, 640, or 1,280 g of the phytase concentrate with 7.920, 7.840, 7.680, 7.360, or 6.720 kg of ground corn. Each of these premixes were added to their respective diets by 0.50% to create final diets containing 250, 500, 1,000, 2,000, or 4,000 FTU/kg
^dAEE Acid hydrolyzed ether extract
^eIP Inositol phosphate. The concentrations of IP3 and inositol in the experimental diets were undetectable

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ISSN: 2049-1891

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