Fig. 7

Inhibition of calcium ions reduces the inflammatory response by regulating the ROS/AMPK/mTOR/autophagy pathway induced by S. maltophilia. MMEC was pretreated with calcium chelating agent EGTA, and then added with S. maltophilia for 6 h before being collected. A Calcium concentration was measured by Flou-4 fluorescence probe. B ROS level was measured by C11-BODIPY fluorescent probe. C and D Level changes of AMPK and mTOR signaling pathways in MMEC. The increase of p-AMPK level and the decrease of p-mTOR level were alleviated by EGTA. E The autophagic level of MMECs was significantly reduced by EGTA pretreatment. F Under EGTA pretreatment, the expression of p-p38 and p-p65 in the p/38MAPK and NF-κB signaling pathways of MMEC was significantly alleviated. G The decrease of tight junction proteins Occludin and Claudin-3 in MMEC was alleviated. H–I Expression of inflammatory cytokines TNF-α and IL-1β in MMECs. The data are presented as mean ± SD. One-way analysis of variance was performed for statistical analysis. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001 indicate significance from each group. ns, no significance