Fig. 5

Transcriptional regulatory networks of GCs during ovarian aging. A A heatmap visualized the significant regulons by SCENIC analysis in ovarian cells. The score of regulation from high to low is indicated by a colour gradient from red to blue. The number in parentheses indicates the number of target genes regulated by this transcription factor. B A heatmap showing different TFs in ovarian cell types by t-test. Red indicates a larger t-value, and blue indicates a smaller t-value. C Violin plot showing feature TF expression in ovarian cells. D t-SNE plots showing the expression of TFs in GCs. The colour indicates the level of expression. E A heatmap demonstrating different TFs in newborn, young and aging goats by t-test. F Violin plot showing expression of SOX4, FOXO1 and HIF1A in GC subtypes from newborn, young and aging goats. G Regulatory network visualizing potential key TFs in downstream target genes. The dot size represents the regulatory weight of TFs