Fig. 2

The composition analyses of the fecal microbial communities between groups that derived from 16S rRNA gene sequencing and metagenomic sequencing data. a Microbial composition at the phylum level. b Microbial composition at the genus level. c Microbial composition at the species level. The groups are represented along the horizontal axis, and relative abundance is denoted by the stack bar charts. d The overlap and unique ASVs observed at three time points (D0, D10, and D21). Each circle represents a specific time point, and the numbers inside the circles indicate the number of ASVs unique to that time point. The overlapping areas between the circles indicate the number of shared ASVs among the time points. The proportion in square brackets below the number is the ratio of the number in a area to the total ASVs number. e The abundance proportion of ASVs shared by the three time points at each time point by the pie charts. f Relative abundance of genera in common. The time pionts are represented along the horizontal axis, and relative abundance is denoted by the stack bar charts. g LEfSe identified significantly different bacterial taxa at phylum level according to the relative abundance among the three time points. h LEfSe identified significantly different bacterial taxa at genus level. i LEfSe identified significantly different bacterial taxa at species level. Taxa in this graph were statistically significant (P < 0.05) and had an LDA Score > 2.5 (phylum level) or 3.5 (genus and species level), which was considered a significant effect size