Rumen microbial degradation of bromoform from red seaweed (Asparagopsis taxiformis) and the impact on rumen fermentation and methanogenic archaea

Table 5 Effect of the inclusion rate (R) of Asparagopsis taxiformis and the type of diet (D) on the in vitro H₂ cumulative production and production rate at different incubation intervals (Exp. 1)

	HC		HF		SEM	P-value
	CTL	AT	CTL	AT	SEM	R	D	R × D
Cumulative H₂, µL
1.5 h	3.91	0.23	0.10	0.06	1.937	0.361	0.331	0.371
6 h	4.69	5.08	0.30	2.88	2.190	0.514	0.166	0.629
12 h	5.20	23.3	0.52	8.66	3.410	0.004	0.020	0.178
24 h	8.38^c	107^a	1.64^d	26.7^b	14.56	0.002	0.015	0.032
72 h	8.83^c	108^a	2.11^d	27.9^b	14.34	0.002	0.014	0.031
H₂ production rate, µL/h
0–1.5 h	2.61	0.15	0.07	0.04	1.291	0.361	0.331	0.371
1.5–6 h	0.17	1.08	0.04	0.63	0.205	0.006	0.191	0.450
6–12 h	0.08	3.03	0.04	0.96	0.540	0.006	0.082	0.094
12–24 h	0.27^c	6.99^a	0.09^d	1.51^b	1.011	0.003	0.021	0.028
24–72 h	0.01	0.02	0.01	0.02	0.010	0.274	0.698	0.726

HC High-concentrate diet, HF High-forage diet, CTL Control (Asparagopsis taxiformis at 0 DM), AT Asparagopsis taxiformis at 2% DM, SEM Standard error of the mean
^a−dValues within a row with different superscripts differ significantly at P < 0.05 due to R × D interaction

ISSN: 2049-1891