Rumen microbial degradation of bromoform from red seaweed (Asparagopsis taxiformis) and the impact on rumen fermentation and methanogenic archaea

Table 4 Effect of the inclusion rate (R) of Asparagopsis taxiformis and the type of diet (D) on the in vitro CH₄ cumulative production and production rate at different incubation intervals (Exp. 1)

	HC		HF		SEM	P-value
	CTL	AT	CTL	AT	SEM	R	D	R × D
Cumulative CH₄, mL
1.5 h	0.08^ab	0.05^bc	0.13^a	0.00^c	0.017	0.001	0.93	0.029
6 h	1.32	0.08	0.77	0.02	0.185	< 0.001	0.137	0.218
12 h	1.95	0.12	1.85	0.03	0.329	< 0.001	0.771	0.984
24 h	3.18	0.20	4.14	0.04	0.833	0.002	0.644	0.517
72 h	8.36	0.52	13.2	0.15	1.833	< 0.001	0.252	0.187
CH₄ production rate, µL/h
0–1.5 h	56.3^ab	33.3^bc	85.2^a	2.30^c	11.50	0.001	0.93	0.029
1.5–6 h	274	5.91	143	3.62	38.70	< 0.001	0.119	0.130
6–12 h	106	7.71	180	1.88	36.60	0.004	0.379	0.307
12–24 h	102	6.38	191	0.41	49.70	0.018	0.427	0.366
24–72 h	108	6.78	189	2.40	30.80	0.001	0.244	0.199

HC High-concentrate diet, HF High-forage diet, CTL Control (Asparagopsis taxiformis at 0 DM), AT Asparagopsis taxiformis at 2% DM, SEM Standard error of the mean
^a−cValues within a row with different superscripts differ significantly at P < 0.05 due to R × D interaction

ISSN: 2049-1891