Rumen microbial degradation of bromoform from red seaweed (Asparagopsis taxiformis) and the impact on rumen fermentation and methanogenic archaea

Table 2 Effect of the inclusion rate (R) of Asparagopsis taxiformis and the type of diet (D) on the in vitro cumulative gas production and production rate at different incubation intervals (Exp. 1)

	HC		HF		SEM	P-value
	CTL	AT	CTL	AT	SEM	R	D	R × D
Cumulative gas production, mL
0.5 h	6.10	4.60	5.60	4.30	0.18	< 0.001	0.046	0.560
1.5 h	15.2	10.9	14.5	10.0	0.40	< 0.001	0.092	0.812
3 h	25.7	19.0	24.3	18.0	0.60	< 0.001	0.079	0.731
6 h	41.5	31.6	37.3	29.0	1.21	< 0.001	0.02	0.505
8 h	52.3	40.8	44.6	36.0	1.65	< 0.001	0.004	0.395
12 h	66.4	52.5	55.2	44.3	1.37	< 0.001	< 0.001	0.309
16 h	83.6	66.9	69.1	55.8	2.19	< 0.001	< 0.001	0.461
24 h	93.3	75.6	77.5	63.2	2.36	< 0.001	< 0.001	0.488
48 h	115	94.0	105	85.9	2.74	< 0.001	0.007	0.654
72 h	130	106	123	102	3.16	< 0.001	0.108	0.772
Gas production rate, mL/h
0–0.5 h	12.23	9.27	11.18	8.65	0.362	< 0.001	0.046	0.560
0.5–1.5 h	9.09	6.23	8.96	5.69	0.300	< 0.001	0.297	0.508
1.5–3 h	6.98	5.39	6.48	5.30	0.312	0.002	0.367	0.523
3–6 h	5.29	4.20	4.34	3.67	0.227	0.004	0.010	0.380
6–8 h	5.37	4.62	3.64	3.51	0.281	0.153	< 0.001	0.293
8–12 h	3.52	2.93	2.66	2.07	0.139	0.002	< 0.001	0.982
12–16 h	4.31	3.59	3.49	2.87	0.260	0.030	0.016	0.851
16–24 h	1.21	1.08	1.05	0.93	0.066	0.088	0.044	0.969
24–48 h	0.91	0.77	1.13	0.94	0.032	< 0.001	< 0.001	0.579
48–72 h	0.62	0.51	0.79	0.66	0.042	0.023	0.005	0.753

HC High-concentrate diet, HF High-forage diet, CTL Control (Asparagopsis taxiformis at 0 DM), AT Asparagopsis taxiformis at 2% DM, SEM Standard error of the mean

ISSN: 2049-1891