Fig. 4

Single cell view and signature of four stomachs. A t-SNE analysis of 22,589 cells from rumen, reticulum, omasum, and abomasum at d 25, with four tissues labeled in left side, and 12 major cell types labeled in right side with different colors. B Dot plot showing the expression of marker genes for the cell types. C The proportion of each cell type among four different tissues. D Dot plot showing the expression of hub genes of Mred, Mpink, Mmagenta, and Mturquoise in 13 cell types; the hub genes of each module were identified through WGCNA analysis (see Fig. 2E). E Average expression of marker genes for monocytes/macrophages, T cells, proliferative cells, and endothelial cells of four tissues across four ages in the transcriptomic data, respectively. Data across four ages were analyzed using one-way analysis of variance with Tukey’s multiple-comparisons test (^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001, ns: no significant)