Fig. 3
IGF2C/T pigs showed muscle fiber hypertrophy and more IMF deposition. A Representative images of loin muscle area, backfat thickness and marbling of IGF2C/T and WT pigs. The yellow dotted line circled loin muscle area and the black bar represented backfat thickness. B H&E staining of the longissimus lumborum (LL), Psoas major (PM), gastrocnemius (GAS), semitendinosus (ST) and backfat (BF) tissues of 270-day-old IGF2C/T and WT pigs. The statistical analysis was presented as histogram in the right panel. Scale bar = 50 μm. n = 3 per group. C Frozen section: Oil red O staining in the LL muscle adipocytes of IGF2C/T and WT pigs at 270 and 370-day-old. The IMF content was presented as histogram in the right panel. Scale bar = 1 mm. 270 d M: WT, n = 5; ED, n = 5; 270 d F: WT, n = 3; ED, n = 4; 370 d M&F: n = 3 per group. D Immunohistochemical double staining of fast-twitch and slow-twitch myofibers in the LL muscle of 270-day-old IGF2C/T and WT pigs. The fast-twitch myofibers were stained with DAB in brown. The slow-twitch myofibers were stained with AEC in red. Scale bar = 50 μm. n = 3 per group. All data were presented as mean ± SEM. *P < 0.05, **P < 0.01, student's t-test