Fig. 1

Generation of IGF2^T/T pigs using CBE3 editor. A Schematic diagram of the strategy to introduce C > T mutation in ZBED6 binding motif within intron 3 of porcine IGF2 by CBE3. The guide RNA sequence was shown in red, with the covering ZBED6 binding motif was boxed in yellow, and the C3071T was underlined. The PAM sequence was shown in green and underlined. B Schematic overview of the production of IGF2^T/T pigs. C The upper panel showed the representative chromatograms of DNA sequences of wild-type IGF2 (WT) and IGF2 with the introduced intron3-C3071T mutation (ED). The ZBED6 binding motif was underlined in red, and the IGF2-intron3-C3071T mutation site was indicated with black dashed. The lower panel showed the DNA sequences of the wild-type and mutant clones, with guide RNA recognition sites shown in red and the PAM sequence in green. The ZBED6 binding site motif is shown in a yellow box. D and E The mRNA expression and protein levels of IGF2 in PEFs. n = 3 per group. F The longissimus lumborum muscle tissue of 7-day-old IGF2^C/T and WT pigs were subjected for ChIP-qPCR analysis. n = 3 per group. All data were presented as mean ± SEM. ^*P < 0.05, ^**P < 0.01, student's t-test