Fig. 2

Gene expression patterns of epithelial cells at different developmental stages. A Proportional Area Chart (Half Circle). Two groups of half circles indicate two DEGs sets of epithelial cells, and the areas represent the number of DEGs. B Fuzzy clustering of expression data at five developmental stages. Purple or red colored lines correspond to genes with high membership value, and y axis represents the normalized expression value from the Mfuzz result. C Monocle trajectory inference traces a path of pesudotime and group types. D The heatmap reveals the relative gene expression level of 3 clusters at 2 branches based on branched expression analysis modeling, combined with the GO/KEGG enriched items for each cluster