Fig. 1

Generation of a stage-specific single-cell atlas of the pig mammary gland. A Histological observations of the mammary gland at five developmental stages. B Statistical analysis of adipocytes area and size. C Schematic workflow for snRNA-seq sequencing. D UMAP visualization of all clusters colored by all cell types. Seven cell clusters were identified in the dataset. E The DEGs analysis shows upregulated genes (Adjusted P value < 0.01) across all seven clusters. F Cell type annotation for all clusters is provided in the bubble chart. G GO annotation and KEGG pathway analysis of differentially expressed genes in each cell-type. H UMAP illustration of cells colored by clusters in separate development stage. I Stacked bar plots represent the proportions of nuclei in the mammary gland