Fig. 4

TM had a non-transgenerational and beneficial effect on heat stroke survival during the first days of life. A Graph showing the theoretical set point temperature (in red) and the actual temperature (in blue) in the rearing cell as a function of time. The period of heat stress due to an uncontrolled heat wave during which mortality was measured is indicated by the double arrow. D: Day of rearing (the first day of post-hatch life being D1). B Stacked histograms showing the number of animals that died or survived between D3 and D6 of rearing during heat stroke. The width of the columns is proportional to the number of animals measured. The C3TM treatment, shown in Fig. 1, corresponds to third generation control animals whose eggs were TM for the first time. Cn: n generations incubated in control conditions; TM2C2: two consecutive generations of TM followed by 2 generations incubated in control conditions. The impact of the heat wave was assessed on quail mortality between D1 and D6 using a Chi2 test, giving a result of P = 0.01088. Different letters indicate significant differences between groups at P < 0.05, obtained by Fisher's tests