Fig. 3
From: Thermal conditioning of quail embryos has transgenerational and reversible long-term effects
TM affects the weight of laid eggs. A Effects of TM on the weight of eggs laid by G1 to G4 quails. Mean and confidence interval were plotted by group at G1–G4 generations. Cn: n generations incubated in control conditions; TMn: n consecutive generations of TM; TM2Cn-2: 2 consecutive generations of TM followed by n-2 generations incubated in control conditions. The results of the general statistical model are available in Additional file 1: Supplementary Tables 2–6, the impact of treatment in the "multig." or "transg." models being always significant (P < 0.05) regardless of the generations of treatment considered. Different letters indicate significant differences between groups at P < 0.05. B Histogram representing the mean egg weight difference laid by TM1, TM2, TM3 or TM4 quails compared to C1, C2, C3 or C4 quails, respectively. C Effects of TM on the weight of eggs laid by G5 to G7 quails. Cn: n generations incubated in control conditions; TM2Cn-2: 2 consecutive generations of TM followed by n-2 generations incubated in control conditions. Different letters indicate significant differences between groups at P < 0.05