Fig. 2

TM affects the growth of hatched quails. A Summary of the statistical results of weight analyses at 4 weeks of age (left) and 5 weeks of age (right). For G1, the 4 weeks measurement has been performed at D31 instead of D28 for all other generations. Complete results including P-values (P) and the effects on the sex and the interaction between treatment and sex can be found in Additional file 1: Supplementary Tables 2–4 and 6. For clarity, results from multigenerational and transgenerational analyses (“multig.” and “transg.”, see methods) are shown on two different lines. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ns: not significant. Color codes (light green to green and light red) reflect the significance of the results (*, **, *** and ns, respectively). The transgenerational effect of TM on weight is circled in purple and the reversion of the effect is circled in red. B–D Effects of thermal manipulation on quail weight at 1 d (B), 4 weeks (C) and 5 weeks (D) of age. Mean and confidence interval were plotted by group at each generation. The weight of quails at D1 is presented independently of sex, this factor having no significant impact (Additional file 1: Supplementary Table 2). For G1, the 4 week measurement has been performed at D31 instead of D28 for all other generations. Cn: n generations incubated in standard control conditions; TMn: n consecutive generations of TM; TM2Cn-2: 2 consecutive generations of TM followed by n-2 generations incubated in control conditions; 1: males; 2: females. Different letters indicate significant differences between groups at P < 0.05