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Table 3 The IVDMD, and fermentation quality of the tested A. oryzae fungal biomass, silage and rapeseed meal ingredients, and the comparisons of the methods at 48h

From: Evaluation of in vitro digestibility of Aspergillus oryzae fungal biomass grown on organic residue derived-VFAs as a promising ruminant feed supplement

Ingredients

GAS

BAG

TT

SEM

P (GAS vs. BAG)

 

AO_ VFAs FWCKM

AO_ VFAs PPL

AO_ Glu.

Hay clover silage

Rapeseed meal

AO_ VFAs FWCKM

AO_ VFAs PPL

AO_ Glu.

Hay clover silage

Rapeseed meal

AO_ VFAs FWCKM

AO_VFAs PPL

AO_Glu.

Hay clover silage

Rapeseed meal

  

IVDMD, %

72.66de

81.44bc

85.94ab

68.80efg

50.9i

69.69de

71.51ef

78.16cd

66.64fg

57.02h

84.11ab

82.33bc

88.63a

65.02g

70.25efg

1.94

0.631

NH4+-N, mg/L

540a

340e

480b

95i

210g

480b

335e

405d

120hi

155h

415d

460bc

430cd

115i

255f

12.82

0.974

Total Gas, mL/g DM

43.81b

39.47cd

53.19a

37.52d

36.58d

26.34e

31.92de

42.43c

19.67f

35.86d

     

3.01

0.763

H2, mL/g DM

5.87bc

6.43b

5.95bc

9.02b

2.34d

5.38bc

5.69bc

4.70c

6.86b

2.05d

     

0.89

0.370

CO2, mL/g DM

34.18a

29.41abc

32.68b

23.34de

21.67de

15.28f

20.26e

28.96bc

12.79f

24.73cd

     

3.2

0.003

CH4, mL/g DM

6.23d

11.40b

14.56a

5.46d

12.58b

5.65d

5.96d

8.75c

0.22e

8.84c

     

0.93

0.188

TVFAs, mmol/L

79.13d

93.28ab

99.23a

84.49bcd

85.32bcd

76.97d

87.30bc

84.61bcd

82.15cd

77.10d

     

3.13

0.003

Acetic, mmol/L

50.00de

58.06b

65.50a

49.40de

49.40de

50.96cd

56.45bc

51.45cd

45.96de

44.13e

     

2.17

0.040

Butyric, mmol/L

18.92d

22.46abcd

20.41cd

27.17ab

25.21abc

22.13bcd

20.32cd

21.95bcd

28.63a

25.31abc

     

2.04

0.553

Propionic, mmol/L

9.15cd

12.46abc

13.32ab

15.25a

10.69bcd

3.88e

10.53bcd

11.20bc

7.55d

7.66d

     

1.21

< 0.001

  1. There are the P-value from the comparison of all methods at 48h (Gas vs. TT vs. Bag on IVDMD (0.042) and NH4+-N (0.928); P-value from the comparison of Gas vs. TT methods on IVDMD (0.232) and NH4+-N (0.984), P-value from the comparison of Gas vs. Bag methods on IVDMD (0.035) and NH4+-N (0.921)
  2. IVDMD: In vitro dry matter digestibility; DM: dry matter; NH4+-N: Ammonium-N; H2: Hydrogen; CO2: Carbon dioxide; CH4: Methane; VFAs: Volatile fatty acids; AO_VFAs FWCKM: A. oryzae biomass grown on VFAs effluents from food waste and chicken manure; AO_VFAs PPL: A. oryzae biomass grown on VFAs effluents from fermented potato protein liquor; AO_Glu.: A. oryzae biomass grown on glucose
  3. Values with different superscript letter in a raw are significantly different at 5% turkey test