Table 2 Sequences of the oligonucleotide primers used for quantitative real-time PCR1
Gene2 | Primer sequence3 (5′→3′) | GenBank accession NO. |
|---|---|---|
AMPK-α1 | F: CGGAGATAAAACAGAAGCACGAG | DQ302133 |
R: CGATTCAGGATCTTCACTGCAAC | ||
Claudin-1 | F: AAGTGCATGGAGGATGACCA | NM_001013611.2 |
R: GCCACTCTGTTGCCATACCA | ||
GAPDH | F: AGAACATCATCCCAGCGTCC | NM_204305 |
R: CGGCAGGTCAGGTCAACAAC | ||
IFN-γ | F: AAAGCCGCACATCAAACACA | NM_205149.1 |
R: GCCATCAGGAAGGTTGTTTTTC | ||
IgA | F: ACCACGGCTCTGACTGTACC | S40610.1 |
R: CGATGGTCTCCTTCACATCA | ||
IL-1β | F: TGGGCATCAAGGGCTACA | NM_204524.1 |
R: CGGCCCACGTAGTAAATGAT | ||
IL-4 | F: GTGCCCACGCTGTGCTTAC | NM_001007079.1 |
R: AGGAAACCTCTCCCTGGATGTC | ||
IL-10 | F: CGCTGTCACCGCTTCTTCA | AJ621614 |
R: TCCCGTTCTCATCCATCTTCTC | ||
IL-17 | F: CTCCGATCCCTTATTCTCCTC | AJ493595 |
R: AAGCGGTTGTGGTCCTCAT | ||
MHC-II | F: CCACGGACGTGATGCAGAAC | 113,206,149 |
R: ACCGCGCAGGAACACGAAGA | ||
NF-κB | F: TGGAGAAGGCTATGCAGCTT | NM_205134.1 |
R: CATCCTGGACAGCAGTGAGA | ||
Occludin | F: AGTTCGACACCGACCTGAAG | NM_205128.1 |
R: TCCTGGTATTGAGGGCTGTC | ||
pIgR | F: ATTTGTCACCACCACAGCCA | NM_001044644 |
R: GAGTAGGCGAGGTCAGCATC | ||
TLR2 | F: ACCTTCTGCACTCTGCCATT | NM_204278.1 |
R: TGTGAATGAAGCACCGGTAA | ||
TLR4 | F: GATGCATCCCCAGTCCGTG | NM_001030693 |
R: CCAGGGTGGTGTTTGGGATT | ||
ZO-1 | F: ACAGCTCATCACAGCCTCCT | XM_015278981.1 |
R: TGAAGGGCTTACAGGAATGG |