Fig. 2

Proteome profiling analysis of different groups. A PCA plot of proteomics (upper) and acetylation (lower). The points represent biological replicates. B Enrichment analysis of DAPs and DEPs. C and D PPI network of DEPs and DAPs between HS0 and HS1 (C) and between HS1 and HS2 (D). The green circles represent the downregulated DEPs, the yellow circles represent the upregulated DEPs, the purple circles represent the hypoacetylated proteins, and the red circles represent the hyperacetylated proteins. E The fat oxidation and primary bile acid biosynthesis pathways were hypoacetylated in the HS2 group compared with the HS1 group. Proteins are labeled by gene symbols with identified acetylation sites. Boxes indicate protein expression (black indicates no significant change in protein expression), circles indicate acetylation sites, and the color scale designates fold-change (HS2/HS1 comparison for protein expression or acetylated site)