Expression of miR-138 in cryopreserved bovine sperm is related to their fertility potential

Salas-Huetos, Albert; Ribas-Maynou, Jordi; Mateo-Otero, Yentel; Tamargo, Carolina; Llavanera, Marc; Yeste, Marc

doi:10.1186/s40104-023-00909-1

Journal of Animal Science and Biotechnology

Table 1 Descriptive data of the population studied: Motility, kinematic parameters and morphology parameters for each fertility group; subfertility group (SF) and high fertility group (HF)

From: Expression of miR-138 in cryopreserved bovine sperm is related to their fertility potential

		Motility and kinematics parameters										Morphology parameters
Animal code	NRR	Total motility, %	Progressive motility, %	VCL, µm/s	VSL, µm/s	VAP, µm/s	LIN, %	STR, %	WOB, %	ALH, µm	BCF, Hz	Normal morphology, %	Cytoplasmic droplets, %	Tail abnormalities, %	Isolated head, %
Subfertility (SF) group
S13	33.33	41.79	11.30	39.19	17.01	21.27	43.41	79.98	54.27	2.38	9.75	93.60	0.45	3.66	2.32
S25	34.58	33.62	19.59	68.26	35.05	42.02	51.07	83.61	61.13	2.53	12.72	86.40	0.00	11.17	2.43
S11	36.25	44.65	18.10	62.77	21.84	29.91	34.77	73.00	47.63	2.97	9.11	88.85	0.00	3.89	5.32
S16	36.40	26.39	14.67	62.14	30.88	37.01	49.66	83.10	59.53	2.18	11.49	92.45	0.00	7.59	0.00
S26	36.52	39.21	19.70	72.70	28.15	37.26	38.71	75.53	51.25	3.17	9.21	90.10	0.00	6.94	1.99
S9	36.86	52.02	25.65	75.20	40.76	47.82	54.22	85.22	63.63	2.89	12.49	85.55	0.50	7.98	3.99
S17	37.60	42.54	21.79	59.51	30.01	34.62	49.98	86.24	57.91	2.76	12.64	84.80	0.00	11.86	3.33
S14	37.65	62.18	31.87	81.61	48.92	55.08	59.94	88.83	67.48	3.25	12.51	91.35	0.00	6.22	1.47
S21	38.25	25.33	16.33	89.56	46.57	52.18	51.90	89.13	58.22	3.64	12.37	94.00	0.00	5.00	1.00
S29	38.43	20.62	14.11	85.78	45.93	50.58	53.52	90.84	58.93	3.36	13.69	84.15	0.91	10.78	3.36
S2	38.84	55.12	21.78	71.05	37.88	43.61	53.22	86.69	61.32	3.29	11.71	88.99	0.96	5.77	2.36
S19	38.98	69.68	43.73	96.46	52.73	58.57	54.77	90.13	60.77	3.65	12.95	88.70	1.00	6.50	1.95
S5	39.11	26.06	20.06	79.64	46.61	51.58	58.52	90.35	64.77	2.29	13.43	94.30	0.50	1.83	3.38
S22	39.34	48.33	29.38	91.40	45.21	52.83	49.46	85.59	57.79	3.59	11.19	88.95	0.48	5.46	2.83
S4	39.34	48.13	35.89	108.85	73.67	77.85	67.56	94.58	71.42	3.29	14.21	89.85	0.36	6.17	3.63
Mean (SD)	37.43 (1.79)	42.38 (14.24)	22.93 (8.90)	76.27 (17.21)	40.08 (13.98)	46.15 (13.56)	51.38 (8.12)	85.52 (5.84)	59.74 (5.98)	3.01 (0.50)	11.96 (1.57)	89.47 (3.28)	0.34 (0.38)	6.72 (2.83)	2.62 (1.30)
High fertility (HF) group
S23	39.51	60.45	32.88	79.56	40.36	46.65	50.69	86.46	58.61	3.33	11.50	86.90	0.00	10.86	2.27
S3	40.03	28.60	17.44	94.55	47.96	54.77	50.44	87.21	57.80	3.71	12.09	92.00	0.00	6.00	0.50
S24	40.13	58.31	37.34	86.15	46.85	54.21	54.23	86.40	62.76	2.92	12.65	88.95	1.43	7.13	1.96
S10	40.13	61.46	41.24	91.84	49.21	55.09	53.49	89.26	59.92	3.41	13.38	89.15	2.95	3.94	3.46
S1	40.29	30.91	20.38	95.74	50.28	55.99	52.40	89.69	58.41	3.92	12.16	94.00	0.00	4.50	1.00
S28	40.33	41.24	24.47	67.02	31.40	37.89	46.26	82.01	56.10	2.34	11.86	84.35	0.00	11.40	2.84
S15	40.37	60.48	35.22	86.43	48.19	53.97	55.72	89.28	62.41	3.27	13.72	85.45	0.00	11.63	2.44
S18	40.60	74.47	48.61	104.17	62.27	73.69	59.75	84.48	70.72	3.21	11.77	91.70	1.47	4.83	1.50
S7	41.06	43.40	29.65	108.27	61.15	67.77	56.48	90.24	62.59	3.67	13.33	91.85	0.47	3.55	2.05
S27	41.34	51.41	31.45	75.89	41.28	47.30	54.50	87.28	62.42	2.69	13.25	90.15	0.99	3.94	4.43
S12	42.28	56.44	40.44	108.14	65.11	71.72	60.14	90.62	66.37	3.34	14.54	81.45	1.92	9.01	6.68
S20	42.34	56.89	27.65	73.56	35.14	41.91	47.84	83.88	57.03	3.36	10.52	89.35	0.48	3.40	5.32
S8	42.94	51.73	28.83	54.89	26.55	31.69	48.42	83.79	57.78	2.25	11.64	86.10	1.49	10.45	1.98
S6	43.48	52.39	28.91	60.90	26.87	33.25	43.98	80.64	54.52	2.42	11.70	91.75	1.45	6.36	0.45
Mean (SD)	41.06 (1.23)	52.01 (12.41)	31.75 (8.41)	84.79 (16.99)	45.19 (12.46	51.85 (13.20)	52.45 (4.82)	86.52 (3.17)	60.53 (4.35)	3.13 (0.53)	12.44 (1.08)	88.80 (3.53)	0.90 (0.92)	6.93 (3.13)	2.63 (1.80)
P-value	< 0.001^b	0.063^a	0.011^a	0.191^a	0.310^a	0.262^a	0.672^a	0.747^b	0.687^a	0.548^a	0.357^a	0.599^a	0.134^b	0.853^a	0.986^a

Data are presented as mean (standard deviation; SD). Data were checked for normal distribution (Shapiro–Wilk test) and homogeneity of variances (Levene test) prior to statistical analysis. Independent T-tests ^a or U-Mann Whitney tests ^b were conducted to determine statistical differences between SF and HF groups with a P-value < 0.05 depending on the normality and homoscedasticity assumptions. Significant values are bolded
Abbreviations: ALH Mean amplitude of the lateral oscillatory movement of the sperm head around the mean trajectory, BCF Number of sperm head lateral oscillatory movements around the mean trajectory per unit of time, HF High fertility group, LIN Linearity coefficient (LIN = VSL/VCL × 100), NRR Non-return rates, RIN RNA integrity number, rRNA Ribosomal RNA, S Svedberg sedimentation coefficient, SD Standard deviation, SF Subfertility group, STR Straightness coefficient (STR = VSL/VAP × 100), VAP Mean sperm trajectory per second, VCL Instantaneous sequential progression along the trajectory, VSL Straight sperm trajectory per second, WOB Wobble coefficient (WOB = VAP/VCL × 100)

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ISSN: 2049-1891

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