Fig. 5

Functional prediction on carbohydrate and amino acid metabolism of cecal microbiota using PICRUSt2. A PCA analysis of KEGG function. B Relative abundance of functional prediction on carbohydrate and amino acid metabolism. C Differences in the functional prediction on carbohydrate metabolism. D Differences in the functional prediction on amino acid metabolism. E Abundance values of butyrate synthesizing enzymes in the cecal microbiota between CON and CTC groups. F Abundance values of butyrate synthesizing enzymes in the cecal microbiota between CON and RE200 groups. CON, control; CTC, 50 mg/kg chlortetracycline; RE200, 200 mg/kg rosemary extract. Values are shown as mean ± SEM, n = 6. ^*P < 0.05