Fig. 7

Effects of oxidative stress induced by H₂O₂ on the Stat3/VEGF-A pathway in porcine vascular endothelial cells (PVECs). A The mRNA expression of angiogenesis-related factors (vascular endothelial growth factor A, VEGF-A; interleukin-6/8, IL-6/8; NADPH oxidase 2, NOX2). B Schematic for the mechanism of H₂O₂-induced angiogenesis impairment. C, D Western blotting analysis of the expression of phospho-Stat3 (p-Stat3), NOX2, and VEGF-A. Cells were treated with 200 μmol/L H₂O₂ for 24 h. Data are presented as mean ± SEM (n = 3). Different letters indicate significant differences at P < 0.05