Fig. 1
From: Development of a vivo rabbit ligated intestinal Loop Model for HCMV infection
PCR detection for HCMV PP65 DNA and HEV ORF2 RNA. Lanes 1\2\3 are samples from control group. Lane 1 was CTDA, Lane 2 was CTSR, Lane 3 was CTVA. We were unable to amplify the DNA of the HCMV pp65and UL16, and RNA of HEV ORF2. Lanes 4\5\6 were samples from double infection group. Lane 4 was DIDA, Lane 5 was DISR, Lane 6 was DIVA. We were successfully amplified all the genes of each lane, especially lane 4 which was from non-injected distal bowel, still showed positive for the injected virus. This indicated that these viruses penetrated into tissue rapidly. Lanes 7\8\9 were samples from single infection (HCMV alone) group. Lane 7 was SIDA, Lane 8 was SISR, Lane 9 was SIVA. We did not detect any signalof HEV RNA. We detected HCMV RNA in the SR and VA but not in DB. GAPDH was an internal loading control