Short-term effect of supplemental yeast extract without or with feed enzymes on growth performance, immune status and gut structure of weaned pigs challenged with Escherichia coli lipopolysaccharide

Table 6 Effect of supplementing AGP, feed enzymes (ENZ) and yeast extract (YE) without or with ENZ on the rectal temperature (°C), concentration of serum (pg/mL) cytokines and fold changes (log₂) of ileal immune cytokines of weaned piglets challenged with E. coli LPS

		LPS treatments^b						Factorial^c			Contrast
Item	NCC^a	AGP	CC	YE	ENZ	YE + ENZ	SEM	Main effect YE	Main effect ENZ	YE × ENZ	NCC vs CC	AGP vs CC	AGP vs YE	AGP vs ENZ	AGP vs YE + ENZ
Rectal temperature
Pre-challenge	38.9	39.2	39.1	39.0	39.2	39.2	0.13	NS	0.06	NS	NS	NS	NS	NS	NS
6 h post-challenge	39.2	39.9	39.9	39.8	40.1	40.1	0.15	NS	NS	NS	0.003	NS	NS	NS	NS
Serum TNF-α
Pre-challenge	98	88	96	77	102	93	9.3	NS	NS	NS	NS	NS	NS	NS	NS
6 h post-challenge	111	1032	1369	854	1621	933	120.8	0.001	NS	NS	<.0001	0.07	0.85	0.003	NS
Serum IL-10
Pre-challenge	18	16	29	22	36	27	15.1	NS	NS	NS	NS	NS	NS	NS	NS
6 h post-challenge	16	157	99	153	86	156	15.3	0.004	NS	NS	0.001	0.02	NS	NS	NS
Ileum cytokines, 96 h post-challenge
IFN-γ	1.00	1.54	1.46	1.51	2.15	0.54	0.152	<.0001	NS	<.0001	0.04	NS	NS	0.008	<.0001
TNF-α	1.00	2.52	2.40	1.37	1.60	1.41	0.244	0.013	0.099	0.073	0.0003	NS	0.002	0.013	0.003
IL-1β	1.00	0.95	1.63	1.02	0.77	0.58	0.154	0.025	0.001	NS	0.01	0.004	NS	NS	NS
IL-6	1.00	0.86	0.85	0.83	0.69	1.12	0.130	NS	NS	NS	NS	NS	NS	NS	NS
IL-10	1.00	0.60	1.31	0.74	0.71	0.76	0.123	0.033	0.019	0.012	0.09	0.0003	NS	NS	NS

^a NCC non-challenged control pigs that were fed the basal diet and injected with sterile saline on d 7
^bPigs fed the basal diet without any additive (CC challenged control) or with AGP, YE, ENZ or ENZ + YE and injected with E. coli LPS on d 7
^c P-value for LPS + ENZ × LPS + YE interaction (CC, YE, ENZ and ENZ + YE)

ISSN: 2049-1891