| −8 wks before parturation | −4 wks before parturation | RP diagnosis weeka | +4 wks after parturition |  | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Item | CON | RP | P-value | CON | RP | P-value | CON | RP | P-value | CON | RP | P-value |
DMI, kg/d | 16.27 ± 0.82 | 12.48 ± 0.88 | 0.02 | 15.92 ± 0.10 | 14.28 ± 0.78 | 0.08 | 20.34 ± 0.56 | 15.55 ± 0.88 | <0.01 | 21.68 ± 0.68 | 20.71 ± 1.34 | 0.53 |
Milk production, kg/d |  |  |  |  |  |  | 30.95 ± 0.32 | 24.61 ± 3.52 | 0.13 | 42.04 ± 2.21 | 38.32 ± 4.23 | 0.45 |
Temperature, °C | 38.23 ± 0.15 | 37.90 ± 0.30 | 0.42 | 38.45 ± 0.08 | 38.42 ± 0.11 | 0.81 | 38.42 ± 0.10 | 38.77 ± 0.16 | 0.09 | 38.32 ± 0.05 | 38.34 ± 0.13 | 0.87 |
BCS | 2.75 ± 0.22 | 3.13 ± 0.22 | 0.18 | 3.04 ± 0.16 | 3.21 ± 0.10 | 0.41 | 3.17 ± 0.17 | 3.17 ± 0.15 | 1.0 | 2.58 ± 0.08 | 2.75 ± 0.14 | 0.41 |
Milk composition, %, unless otherwise stated | ||||||||||||
Fat |  |  |  |  |  |  | 5.08 ± 0.45 | 4.29 ± 0.44 | 0.25 |  |  |  |
Protein |  |  |  |  |  |  | 3.00 ± 0.10 | 3.12 ± 0.13 | 0.50 |  |  |  |
Fat : Protein ratio |  |  |  |  |  |  | 1.69 ± 0.12 | 1.38 ± 0.14 | 0.13 |  |  |  |
Lactose |  |  |  |  |  |  | 4.54 ± 0.05 | 4.38 ± 0.05 | 0.05 |  |  |  |
SCC, 103 cells/mL |  |  |  |  |  |  | 28.33 ± 5.63 | 108.67 ± 39.46 | 0.10 |  |  |  |
Milk urea N, mg/dL |  |  |  |  |  |  | 15.39 ± 0.76 | 13.33 ± 1.66 | 0.29 |  |  |  |
TS |  |  |  |  |  |  | 12.21 ± 0.31 | 13.98 ± 1.14 | 0.19 |  |  |  |
Serum parameters | ||||||||||||
Lactate, μmol/L | 2,455 ± 348 | 4,855 ± 692 | 0.05 | 2,162 ± 184 | 5,507 ± 933 | 0.03 | 2,227 ± 320 | 4,458 ± 925 | 0.05 | 2,100 ± 129 | 3,726 ± 468 | 0.04 |
NEFA, mmol/L | 140.79 ± 32.77 | 193.32 ± 54.25 | 0.41 | 194 ± 47 | 182 ± 37.61 | 0.87 | 756.51 ± 232.01 | 471 ± 146 | 0.34 | 500.43 ± 151.71 | 269.48 ± 69.13 | 0.23 |
BHBA, μmol/L | 352 ± 37.71 | 340 ± 63.99 | 0.87 | 312 ± 18 | 366 ± 49.62 | 0.30 | 827 ± 151.50 | 553 ± 85 | 0.15 | 896.73 ± 188.3 | 553.15 ± 85.24 | 0.13 |
IL-1, pg/mL | 317 ± 6.04 | 347 ± 15.90 | 0.04 | 321 ± 1.59 | 337. ± 6.04 | 0.02 | 277 ± 5.42 | 290 ± 7.36 | 0.05 | 270.57 ± 2.87 | 287.51 ± 8.99 | 0.05 |
IL-6, pg/mL | 19.23 ± 5.67 | 100 ± 41.45 | 0.02 | 48.24 ± 17.51 | 69.39 ± 18.43 | 0.34 | 23.17 ± 5.18 | 35.65 ± 21.63 | 0.59 | 15.71 ± 3.27 | 32.99 ± 7.32 | 0.10 |
TNF-α, ng/mL | 0.34 ± 0.03 | 1.26 ± 0.30 | 0.03 | 0.27 ± 0.05 | 1.31 ± 0.22 | <0.01 | 0.06 ± 0.03 | 1.37 ± 0.41 | 0.01 | 0.07 ± 0.04 | 1.04 ± 0.25 | 0.05 |
Haptoglobin, mg/mL | 0.19 ± 0.03 | 0.34 ± 0.25 | 0.58 | 0.15 ± 0.01 | 0.07 ± 0.02 | <0.01 | 0.12 ± 0.01 | 1.06 ± 0.31 | <0.01 | 0.16 ± 0.01 | 0.17 ± 0.17 | 0.98 |
SAA, ug/mL | 8,447 ± 3373 | 24,584 ± 7,794 | 0.04 | 3,461 ± 342 | 19,378 ± 6,445 | 0.03 | 10,401 ± 1,722 | 21,706 ± 8,746 | 0.04 | 11,797 ± 1,853 | 23,955 ± 10,369 | <0.01 |