Fig. 1From: Optimized production of transgenic buffalo embryos and offspring by cytoplasmic zygote injectionCharacterization of EGFP-positive buffalo blastocysts. a. Microscopic evaluation by brightfield (a) and wide-field epifluorescence (a’); scale bar =100 μm. b. Nuclei numbers in EGFP-positive and non-injected IVF blastocysts. c. Mixed-sex twin buffalo calves born in December 2010Back to article page