Impact of source tissue and ex vivo expansion on the characterization of goat mesenchymal stem cells

Table 2 Primer sequences used in quantitative RT-PCR

Gene	Primer sequences (5’ → 3’)	Product size, bp	Anneal T, °C	Source
GAPDH	F: TTGTGATGGGCGTGAACC	127	60	[37]
GAPDH	R: CCCTCCACGATGCCAAA	127	60	[37]
PPARG	F: AAAGCGTCAGGGTTCCACTA	201	60	[38]
PPARG	R: CCCGAACCTGATGGCGTTAT	201	60	[38]
FABP4	F: TGAGATGTCCTTCAAATTGGG	101	60	[39]
FABP4	R: CTTGTACCAGAGCACCTTCATC	101	60	[39]
CD90	F: GAATCCCACCGTCTCCAATA	190	60	XM_005689553.1
(THY1)	R: CTTGTGGCTTCCTGTGTCCT	190	60	XM_005689553.1
CD73	F: CTGAGACACCCGGATGAGAT	160	57	NM_174129.3
(NT5E)	R: ACTGGACCAGGTCAAAGGTG	160	57	NM_174129.3
CD105	F: GCAAAGCATCTTCCTTCGTC	56	60	NM_001076397.1
(ENG)	R: GATTGCAGGAGAACGGTGAG

The sequences of primers used are listed with the genes amplified, PCR product sizes (bp), annealing temperatures and sources. Primers were acquired from the indicated references, or designed using Primer3 based on the GenBank accessions provided.

ISSN: 2049-1891